Correction to: Characterization of the first fully human anti-TEM1 scFv in models of solid tumor imaging and immunotoxin-based therapy.

Détails

ID Serval
serval:BIB_EF0FC7F11707
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Correction to: Characterization of the first fully human anti-TEM1 scFv in models of solid tumor imaging and immunotoxin-based therapy.
Périodique
Cancer immunology, immunotherapy
Auteur(s)
Yuan X., Yang M., Chen X., Zhang X., Sukhadia S., Musolino N., Bao H., Chen T., Xu C., Wang Q., Santoro S., Ricklin D., Hu J., Lin R., Yang W., Li Z., Qin W., Zhao A., Scholler N., Coukos G.
ISSN
1432-0851 (Electronic)
ISSN-L
0340-7004
Statut éditorial
Publié
Date de publication
02/2018
Peer-reviewed
Oui
Volume
67
Numéro
2
Pages
329-339
Langue
anglais
Notes
Publication types: Published Erratum
Publication Status: ppublish
Résumé
Tumor endothelial marker 1 (TEM1) has been identified as a novel surface marker upregulated on the blood vessels and stroma in many solid tumors. We previously isolated a novel single-chain variable fragment (scFv) 78 against TEM1 from a yeast display scFv library. Here we evaluated the potential applications of scFv78 as a tool for tumor molecular imaging, immunotoxin-based therapy and nanotherapy. Epitope mapping, three-dimensional (3D) structure docking and affinity measurements indicated that scFv78 could bind to both human and murine TEM1, with equivalent affinity, at a well-conserved conformational epitope. The rapid internalization of scFv78 and scFv78-labeled nanoparticles was triggered after specific TEM1 binding. The scFv78-saporin immunoconjugate also exerted dose-dependent cytotoxicity with high specificity to TEM1-positive cells in vitro. Finally, specific and sensitive tumor localization of scFv78 was confirmed with optical imaging in a mouse tumor model that has highly endogenous mTEM1 expression in the vasculature. Our data indicate that scFv78, the first fully human anti-TEM1 recombinant antibody, recognizes both human and mouse TEM1 and has unique and favorable features that are advantageous for the development of imaging probes or antibody-toxin conjugates for a large spectrum of human TEM1-positive solid tumors.

Mots-clé
Immunotoxin, Nanoparticle, Optical imaging, ScFv, TEM1
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/01/2018 12:00
Dernière modification de la notice
09/05/2019 3:11
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