Increased Resistance to Carbapenems in Proteus mirabilis Mediated by Amplification of the bla<sub>VIM-1</sub>-Carrying and IS26-Associated Class 1 Integron.
Details
Serval ID
serval:BIB_EEBEAEDE7BA4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Increased Resistance to Carbapenems in Proteus mirabilis Mediated by Amplification of the bla<sub>VIM-1</sub>-Carrying and IS26-Associated Class 1 Integron.
Journal
Microbial drug resistance
ISSN
1931-8448 (Electronic)
ISSN-L
1076-6294
Publication state
Published
Issued date
06/2019
Peer-reviewed
Oui
Volume
25
Number
5
Pages
663-667
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Objective:
The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.
Methods:
The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.
Results:
A series of clinical P. mirabilis isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of bla <sub>VIM-1</sub> gene copies.
Conclusion:
We showed here that increased carbapenem resistance in P. mirabilis may result from increased expression of the bla <sub>VIM-1</sub> carbapenemase gene through multiplication of its copy number.
The aim of the study was to decipher the mechanisms and associated genetic determinants responsible for increased carbapenem resistance among Proteus mirabilis clinical isolates.
Methods:
The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking, and DNA sequencing.
Results:
A series of clinical P. mirabilis isolates were consecutively recovered from different patients at the Military hospital of Sofia, Bulgaria. They showed variable levels of resistance to carbapenems. All isolates produced the same carbapenemase VIM-1 that was chromosomally encoded. We showed that increased resistance to carbapenems was related to an increased number of bla <sub>VIM-1</sub> gene copies.
Conclusion:
We showed here that increased carbapenem resistance in P. mirabilis may result from increased expression of the bla <sub>VIM-1</sub> carbapenemase gene through multiplication of its copy number.
Keywords
Anti-Bacterial Agents/pharmacology, Bulgaria/epidemiology, Carbapenems/pharmacology, Chromosomes, Bacterial/chemistry, Chromosomes, Bacterial/metabolism, Gene Dosage, Gene Expression, Hospitals, Humans, Integrons, Military Personnel, Proteus Infections/drug therapy, Proteus Infections/epidemiology, Proteus Infections/microbiology, Proteus mirabilis/drug effects, Proteus mirabilis/enzymology, Proteus mirabilis/genetics, Proteus mirabilis/isolation & purification, Sequence Analysis, DNA, beta-Lactam Resistance/genetics, beta-Lactamases/genetics, beta-Lactamases/metabolism, IS, VIM-1, carbapenem resistance
Pubmed
Web of science
Create date
01/03/2019 12:33
Last modification date
16/12/2019 6:19
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