Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.

Détails

ID Serval
serval:BIB_EE873B8A0DD8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.
Périodique
Journal of Chromatography. B, Analytical Technologies In the Biomedical and Life Sciences
Auteur(s)
Varlet V., Smith F., Augsburger M.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Statut éditorial
Publié
Date de publication
2013
Volume
913-914
Pages
155-160
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The aim of our study was to provide an innovative HS-GC/MS method applicable to the routine determination of butane concentration in forensic toxicology laboratories. The main drawback of the GC/MS methods discussed in literature concerning butane measurement was the absence of a specific butane internal standard necessary to perform quantification. Because no stable isotope of butane is commercially available, it is essential to develop a new approach by an in situ generation of standards. To avoid the manipulation of a stable isotope-labelled gas, we have chosen to generate in situ an internal labelled standard gas (C(4)H(9)D) following the basis of the stoichiometric formation of butane by the reaction of deuterated water (D(2)O) with Grignard reagent butylmagnesium chloride (C(4)H(9)MgCl). This method allows a precise measurement of butane concentration and therefore, a full validation by accuracy profile was presented.
Mots-clé
In situ stable labelled isotope generation, Butane, Deuterated butane, Grignard reagent, Headspace gas extraction
Pubmed
Web of science
Création de la notice
07/03/2013 19:29
Dernière modification de la notice
03/03/2018 22:33
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