Immunohistochemical detection of fibroblast growth factor receptors in normal endocrine cells and related tumors of the digestive system.

Détails

ID Serval
serval:BIB_EE67BE92357C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Immunohistochemical detection of fibroblast growth factor receptors in normal endocrine cells and related tumors of the digestive system.
Périodique
Applied Immunohistochemistry and Molecular Morphology : Aimm / Official Publication of the Society For Applied Immunohistochemistry
Auteur(s)
La Rosa S., Uccella S., Erba S., Capella C., Sessa F.
ISSN
1541-2016 (Print)
ISSN-L
1533-4058
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
9
Numéro
4
Pages
319-328
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Endocrine tumors (ETs) of the digestive system produce several growth factors including acidic and basic fibroblast growth factors (aFGF and bFGF, respectively), which are thought to be involved in the growth of tumor cells and in the proliferation of tumor stromal cells. Their actions depend on binding to four specific receptors--FGFR1, FGFR2, FGFR3, and FGFR4--whose distribution in normal endocrine cells and related tumors of the gastroenteropancreatic (GEP) system has previously been examined. Formalin-fixed, paraffin-embedded normal tissues and 60 well-characterized GEP endocrine tumors were immunostained using specific antibodies directed against various GEP hormones, aFGF, FGFR1, FGFR2, FGFR3, and FGFR4. Acidic FGF immunoreactivity (IR) was found in gut EC cells; FGFR1 immunoreactivity in rare duodenal endocrine cells and in pancreatic A cells; FGFR2 immunoreactivity in gastric and duodenal G cells, pancreatic B cells, and rectal EC cells; FGFR3 immunoreactivity in duodenal G cells; and FGFR4 immunoreactivity in rectal L cells and in pancreatic B, PP, and A cells. Immunoreactivity for at least one of the four FGFRs was found in all tumors, independently of FGFR expression in the putative cell of origin. EC cell tumors, which were all positive for aFGF, were found to express at least three different FGFRs. FGFRs also were localized in the stromal cells of all the tumors examined. The tumor stroma was more abundant in EC cell tumors than in other types of neoplasms. The results suggest that aFGF-FGFR interaction may be involved in the modulation of normal endocrine cell functions and in the regulation of tumor growth and stromal proliferation of EC cell carcinoids.
Mots-clé
Adolescent, Adult, Aged, Aged, 80 and over, Diagnostic Techniques, Endocrine, Digestive System/chemistry, Digestive System/cytology, Digestive System Neoplasms/chemistry, Digestive System Neoplasms/pathology, Endocrine Gland Neoplasms/chemistry, Endocrine Gland Neoplasms/pathology, Enteroendocrine Cells/chemistry, Enteroendocrine Cells/cytology, Female, Humans, Immunohistochemistry, Intestinal Neoplasms/chemistry, Intestinal Neoplasms/pathology, Male, Middle Aged, Pancreatic Neoplasms/chemistry, Pancreatic Neoplasms/pathology, Protein-Tyrosine Kinases, Receptor Protein-Tyrosine Kinases/metabolism, Receptor, Fibroblast Growth Factor, Type 1, Receptor, Fibroblast Growth Factor, Type 2, Receptor, Fibroblast Growth Factor, Type 3, Receptor, Fibroblast Growth Factor, Type 4, Receptors, Fibroblast Growth Factor/metabolism, Tissue Distribution
Pubmed
Web of science
Création de la notice
07/09/2016 8:29
Dernière modification de la notice
20/08/2019 16:15
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