Adaptation of canine distemper virus to canine footpad keratinocytes modifies polymerase activity and fusogenicity through amino acid substitutions in the P/V/C and H proteins.

Détails

ID Serval
serval:BIB_EE59F488572A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Adaptation of canine distemper virus to canine footpad keratinocytes modifies polymerase activity and fusogenicity through amino acid substitutions in the P/V/C and H proteins.
Périodique
Virology
Auteur(s)
Rivals J.P., Plattet P., Currat-Zweifel C., Zurbriggen A., Wittek R.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Statut éditorial
Publié
Date de publication
03/2007
Volume
359
Numéro
1
Pages
6-18
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The wild-type canine distemper virus (CDV) strain A75/17 induces a non-cytocidal infection in cultures of canine footpad keratinocytes (CFKs) but produces very little progeny virus. After only three passages in CFKs, the virus produced 100-fold more progeny and induced a limited cytopathic effect. Sequence analysis of the CFK-adapted virus revealed only three amino acid differences, of which one was located in each the P/V/C, M and H proteins. In order to assess which amino acid changes were responsible for the increase of infectious virus production and altered phenotype of infection, we generated a series of recombinant viruses. Their analysis showed that the altered P/V/C proteins were responsible for the higher levels of virus progeny formation and that the amino acid change in the cytoplasmic tail of the H protein was the major determinant of cytopathogenicity.
Mots-clé
Adaptation, Biological/genetics, Amino Acid Substitution, Animals, Cell Line, Cells, Cultured, Cytopathogenic Effect, Viral/genetics, DNA Mutational Analysis, DNA-Directed RNA Polymerases/metabolism, Distemper Virus, Canine/genetics, Distemper Virus, Canine/growth & development, Dogs, Keratinocytes/virology, Mutation, Missense, Proteins, Recombination, Genetic, Viral Plaque Assay, Viral Proteins/chemistry, Viral Proteins/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 16:15
Données d'usage