Article: article from journal or magazin.
Generation of cytolytic T lymphocytes in vitro. XII. Detection of memory CTL precursors in peripheral blood of alloimmune mice
Scandinavian Journal of Immunology
The memory cytolytic T lymphocyte (CTL) response of mice was studied in vitro using peripheral blood leucocytes (PBL) of donors previously immunized with allogeneic cells as responding cells, and particulate alloantigen for stimulation. It is shown that alloimmune but not normal PBL respond to particulate antigen and that small numbers of PBL generate high CTL responses provided non-T accessory spleen cells are added to the cultures. The system provides means to analyse the kinetics of the appearance of memory CTL precursors after primary alloimmunization in individual mice.
Animals Cells, Cultured Immunization *Immunologic Memory Isoantibodies Isoantigens Mice Mice, Inbred C57BL Mice, Inbred DBA Spleen/cytology T-Lymphocytes/*immunology
Web of science
Last modification date