Production of tumor necrosis factor-alpha by naive or memory T lymphocytes activated via CD28

Details

Serval ID
serval:BIB_EDE80937E561
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Production of tumor necrosis factor-alpha by naive or memory T lymphocytes activated via CD28
Journal
Cellular Immunology
Author(s)
von Fliedner  V., Miescher  S., Gerain  J., Gallati  H., Barras  C., Heumann  D., Cerottini  J. C.
ISSN
0008-8749 (Print)
Publication state
Published
Issued date
01/1992
Volume
139
Number
1
Pages
198-207
Notes
In Vitro
Journal Article --- Old month value: Jan
Abstract
While it is well established that activated T cells can produce tumor necrosis factor alpha (TNF-alpha), it is less clear whether this function is confined to a given subset, e.g., memory cells. To approach this question, we investigated the production of TNF-alpha by human peripheral blood T lymphocytes activated with anti-CD28 mAb since this activation pathway is known to potentiate cytokine production. Under the culture conditions used, the amount of TNF-alpha produced was markedly enhanced compared to that obtained after activation with immobilized anti-CD3 mAb. The enhancement of TNF-alpha production was already apparent after incubation of T cells for 6 hr. Up to 5 ng/ml of TNF-alpha was measured on Day 2 in supernatants of cultures of 10(4) T lymphocytes. To determine the source of the cells producing high amounts of TNF-alpha, T lymphocytes were separated into two subpopulations, namely naive cells (expressing the CD45RA isoform) and memory cells (expressing the CD45RO isoform). While both subpopulations proliferated equally well after stimulation with anti-CD28 mAb, up to 90% of the TNF-alpha produced under these conditions originated from memory T cells. These results thus document that T cell activation via CD28 results in a marked increase in TNF-alpha production without affecting the functional disparity that exists between naive and memory T cells.
Keywords
Antibodies, Monoclonal Antigens, CD/analysis/*immunology Antigens, CD28 Antigens, CD45 Antigens, Differentiation, T-Lymphocyte/*immunology Histocompatibility Antigens/analysis Humans Immunologic Memory Interferon Type II/biosynthesis *Lymphocyte Activation T-Lymphocyte Subsets/*immunology Tetradecanoylphorbol Acetate/pharmacology Time Factors Tumor Necrosis Factor-alpha/*biosynthesis
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 16:15
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