Proteasome-assisted identification of a SSX-2-derived epitope recognized by tumor-reactive CTL infiltrating metastatic melanoma.
Details
Serval ID
serval:BIB_EDDA88773A60
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteasome-assisted identification of a SSX-2-derived epitope recognized by tumor-reactive CTL infiltrating metastatic melanoma.
Journal
Journal of Immunology
ISSN
0022-1767[print], 0022-1767[linking]
Publication state
Published
Issued date
2002
Volume
168
Number
4
Pages
1717-1722
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
The tumor Ag SSX-2 (HOM-MEL-40) was found by serological identification of Ags by recombinant expression cloning and was shown to be a cancer/testis Ag expressed in a wide variety of tumors. It may therefore represent a source of CD8(+) T cell epitopes useful for specific immunotherapy of cancer. To identify potential SSX-2-derived epitopes that can be recognized by CD8(+) T cells, we used an approach that combined: 1) the in vitro proteasomal digestion of precursor peptides overlapping the complete SSX-2 sequence; 2) the prediction of SSX-2-derived peptides with an appropriate HLA-A2 binding score; and 3) the analysis of a tumor-infiltrated lymph node cell population from an HLA-A2(+) melanoma patient with detectable anti-SSX-2 serum Abs. This strategy allowed us to identify peptide SSX-2(41-49) as an HLA-A2-restricted epitope. SSX2(41-49)-specific CD8(+) T cells were readily detectable in the tumor-infiltrated lymph node population by multimer staining, and CTL clones isolated by multimer-guided cell sorting were able to lyse HLA-A2(+) tumor cells expressing SSX-2.
Keywords
Amino Acid Sequence, Animals, Antigens, Neoplasm/chemistry, Antigens, Neoplasm/immunology, COS Cells, Clone Cells, Cysteine Endopeptidases/metabolism, Cytotoxicity Tests, Immunologic, Epitope Mapping, Epitopes, T-Lymphocyte/immunology, HLA-A2 Antigen/physiology, Humans, Lymphatic Metastasis, Lymphocytes, Tumor-Infiltrating/immunology, Melanoma/immunology, Melanoma/pathology, Molecular Sequence Data, Multienzyme Complexes/metabolism, Neoplasm Proteins/chemistry, Neoplasm Proteins/immunology, Peptide Fragments/immunology, Peptides/immunology, Proteasome Endopeptidase Complex, Repressor Proteins/chemistry, Repressor Proteins/immunology, T-Lymphocytes, Cytotoxic/immunology, Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 12:17
Last modification date
20/08/2019 17:15