Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death

Details

Serval ID
serval:BIB_EDD4BE6837D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death
Journal
Journal of Clinical Investigation
Author(s)
French  L. E., Wohlwend  A., Sappino  A. P., Tschopp  J., Schifferli  J. A.
ISSN
0021-9738 (Print)
Publication state
Published
Issued date
02/1994
Volume
93
Number
2
Pages
877-84
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
Clusterin is a serum glycoprotein endowed with cell aggregating, complement inhibitory, and lipid binding properties, and is also considered as a specific marker of dying cells, its expression being increased in various tissues undergoing programmed cell death (PCD). However, no study has so far directly shown that cells expressing clusterin in these tissues are actually apoptotic as defined by morphological and biochemical criteria. We have studied cellular clusterin gene expression in vitro using three different models of PCD: (a) ultraviolet B (UV-B) irradiation of human U937, HeLa, and A431 cell lines, (b) in vitro aging of human peripheral blood neutrophils (PMNs), and (c) dexamethasone-induced cell death of the human lymphoblastoid cell line CEM-C7. In all three models, the classical morphological and biochemical features of PCD observed did not correlate with an increase, but with either a marked decrease or an absence of clusterin gene expression as assessed by Northern blot analysis. In situ hybridization of U937 and A431 cells after UV-B irradiation revealed, in addition, that only morphologically normal cells that are surviving continue to express the clusterin gene. Our results demonstrate that in the human myeloid, lymphoid, and epithelial cell types studied, clusterin gene expression is not a prerequisite to their death by apoptosis. In addition, and most interestingly, in situ hybridization of U937 and A431 cells revealed that only surviving cells express the clusterin gene after the induction of PCD, thus providing novel evidence suggesting that clusterin may be associated with cell survival within tissues regressing as a consequence of PCD.
Keywords
*Apoptosis/drug effects Blotting, Northern Carcinoma, Squamous Cell Cell Aging Cell Line Cell Survival/drug effects/*physiology Clusterin Dexamethasone/pharmacology *Gene Expression Glycoproteins/*biosynthesis Hela Cells Humans In Situ Hybridization *Molecular Chaperones Neoplasm Proteins/biosynthesis Neutrophils/*cytology/*metabolism RNA Probes RNA, Messenger/analysis/biosynthesis Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:18
Last modification date
20/08/2019 16:15
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