Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation.

Détails

ID Serval
serval:BIB_ED9F802C1F1F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mammalian RNA Decay Pathways Are Highly Specialized and Widely Linked to Translation.
Périodique
Molecular cell
Auteur(s)
Tuck A.C., Rankova A., Arpat A.B., Liechti L.A., Hess D., Lesmantavicius V., Castelo-Szekely V., Gatfield D., Bühler M.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Statut éditorial
In Press
Peer-reviewed
Oui
Pages
1-15
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Résumé
RNA decay is crucial for mRNA turnover and surveillance and misregulated in many diseases. This complex system is challenging to study, particularly in mammals, where it remains unclear whether decay pathways perform specialized versus redundant roles. Cytoplasmic pathways and links to translation are particularly enigmatic. By directly profiling decay factor targets and normal versus aberrant translation in mouse embryonic stem cells (mESCs), we uncovered extensive decay pathway specialization and crosstalk with translation. XRN1 (5'-3') mediates cytoplasmic bulk mRNA turnover whereas SKIV2L (3'-5') is universally recruited by ribosomes, tackling aberrant translation and sometimes modulating mRNA abundance. Further exploring translation surveillance revealed AVEN and FOCAD as SKIV2L interactors. AVEN prevents ribosome stalls at structured regions, which otherwise require SKIV2L for clearance. This pathway is crucial for histone translation, upstream open reading frame (uORF) regulation, and counteracting ribosome arrest on small ORFs. In summary, we uncovered key targets, components, and functions of mammalian RNA decay pathways and extensive coupling to translation.
Mots-clé
Cell Biology, Molecular Biology, AVEN, RNA, RNA decay, RNA degradation, RNA surveillance, SKIV2L, histones, ribosome, ribosome stalling, translation
Pubmed
Open Access
Oui
Financement(s)
Fonds national suisse / Projets / 51NF40_182880
Fondation Novartis
Université de Lausanne
Fonds national suisse / Projets / 31003A_179190
Création de la notice
12/02/2020 15:08
Dernière modification de la notice
15/02/2020 7:18
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