Different transcriptional control of metabolism and extracellular matrix in visceral and subcutaneous fat of obese and rimonabant treated mice.

Détails

Ressource 1Télécharger: BIB_ED5FF5045C10.P001.pdf (743.69 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_ED5FF5045C10
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Different transcriptional control of metabolism and extracellular matrix in visceral and subcutaneous fat of obese and rimonabant treated mice.
Périodique
PLoS ONE
Auteur(s)
Poussin C., Hall D., Minehira K., Galzin A.M., Tarussio D., Thorens B.
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
3
Numéro
10
Pages
e3385
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
BACKGROUND: The visceral (VAT) and subcutaneous (SCAT) adipose tissues play different roles in physiology and obesity. The molecular mechanisms underlying their expansion in obesity and following body weight reduction are poorly defined. METHODOLOGY: C57Bl/6 mice fed a high fat diet (HFD) for 6 months developed low, medium, or high body weight as compared to normal chow fed mice. Mice from each groups were then treated with the cannabinoid receptor 1 antagonist rimonabant or vehicle for 24 days to normalize their body weight. Transcriptomic data for visceral and subcutaneous adipose tissues from each group of mice were obtained and analyzed to identify: i) genes regulated by HFD irrespective of body weight, ii) genes whose expression correlated with body weight, iii) the biological processes activated in each tissue using gene set enrichment analysis (GSEA), iv) the transcriptional programs affected by rimonabant. PRINCIPAL FINDINGS: In VAT, "metabolic" genes encoding enzymes for lipid and steroid biosynthesis and glucose catabolism were down-regulated irrespective of body weight whereas "structure" genes controlling cell architecture and tissue remodeling had expression levels correlated with body weight. In SCAT, the identified "metabolic" and "structure" genes were mostly different from those identified in VAT and were regulated irrespective of body weight. GSEA indicated active adipogenesis in both tissues but a more prominent involvement of tissue stroma in VAT than in SCAT. Rimonabant treatment normalized most gene expression but further reduced oxidative phosphorylation gene expression in SCAT but not in VAT. CONCLUSION: VAT and SCAT show strikingly different gene expression programs in response to high fat diet and rimonabant treatment. Our results may lead to identification of therapeutic targets acting on specific fat depots to control obesity.
Mots-clé
Abdominal Fat, Adipocytes, Animals, Blood Glucose, Body Weight, Dietary Fats, Extracellular Matrix, Gene Expression Regulation, Insulin, Leptin, Lipoproteins, VLDL, Mice, Mice, Inbred C57BL, Obesity, Piperidines, Pyrazoles, Receptors, Cannabinoid, Subcutaneous Fat, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2009 22:12
Dernière modification de la notice
20/08/2019 16:15
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