Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.

Détails

ID Serval
serval:BIB_ED2E119D0D00
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.
Périodique
Cell
Auteur(s)
Hernandez N., Keller W.
ISSN
0092-8674 (Print)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
11/1983
Volume
35
Numéro
1
Pages
89-99
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Using a whole cell extract of HeLa cells, we synthesized unspliced RNAs containing the first two leaders and the first intervening sequence of the adenovirus 2 major late transcription unit. Upon incubation of these pre-mRNAs in reaction mixtures containing a nuclear extract and a postnuclear fraction (S100), removal of the first intervening sequence and concomitant joining of the first leader to the second leader was observed. This splicing reaction requires proteins, Mg2+ ions, and ATP. The S100 fraction alone has no splicing activity but stimulates splicing when added to the nuclear extract. Upon fractionation of the postnuclear S100 by chromatography on ion exchange and gel filtration columns, the stimulatory activity copurifies with small ribonucleoprotein particles.
Mots-clé
Adenosine Triphosphate/pharmacology, Adenoviruses, Human/analysis, Base Sequence, Cell Extracts, Cell Fractionation, Cell Nucleus/analysis, HeLa Cells, Humans, Magnesium/pharmacology, Nucleic Acid Precursors/metabolism, RNA/metabolism, RNA Precursors, RNA Splicing, RNA, Messenger/metabolism, RNA, Viral/metabolism, Ribonucleoproteins/isolation & purification, Ribonucleoproteins/physiology, Ribonucleoproteins, Small Nuclear
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 17:15
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