Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.

Détails

ID Serval
serval:BIB_EC4BA54639FF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.
Périodique
ACS Nano
Auteur(s)
Hansel C.S., Crowder S.W., Cooper S., Gopal S., João Pardelha da Cruz M., de Oliveira Martins L., Keller D., Rothery S., Becce M., Cass AEG, Bakal C., Chiappini C., Stevens M.M.
ISSN
1936-086X (Electronic)
ISSN-L
1936-0851
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
13
Numéro
3
Pages
2913-2926
Langue
anglais
Résumé
Biomaterial substrates can be engineered to present topographical signals to cells which, through interactions between the material and active components of the cell membrane, regulate key cellular processes and guide cell fate decisions. However, targeting mechanoresponsive elements that reside within the intracellular domain is a concept that has only recently emerged. Here, we show that mesoporous silicon nanoneedle arrays interact simultaneously with the cell membrane, cytoskeleton, and nucleus of primary human cells, generating distinct responses at each of these cellular compartments. Specifically, nanoneedles inhibit focal adhesion maturation at the membrane, reduce tension in the cytoskeleton, and lead to remodeling of the nuclear envelope at sites of impingement. The combined changes in actin cytoskeleton assembly, expression and segregation of the nuclear lamina, and localization of Yes-associated protein (YAP) correlate differently from what is canonically observed upon stimulation at the cell membrane, revealing that biophysical cues directed to the intracellular space can generate heretofore unobserved mechanosensory responses. These findings highlight the ability of nanoneedles to study and direct the phenotype of large cell populations simultaneously, through biophysical interactions with multiple mechanoresponsive components.
Mots-clé
cell−material interactions, mechanotransduction, nanoneedles, nuclear mechanics, porous silicon, super-resolution microscopy
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/04/2019 13:12
Dernière modification de la notice
20/08/2019 17:14
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