Microcolony growth procedures have gained recent interest for the following reasons: rapid simultaneous screening for growth of large numbers of bacteria from environmental samples, time-lapse imaging of single-cell growth, cell aging or biofilm studies. Not many of such procedures have been described in sufficient technical detail to be easily reproduced. Here we provide a simple step-by-step procedure, which allows time-lapse imaging of bacterial cell division and cell tracking up to the stage of a monolayered colony of a few 100 cells.