Using BUSCO to Assess Insect Genomic Resources

Détails

Ressource 1Télécharger: MiMB-Waterhouse-BUSCO_Serval.pdf (1084.80 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_EBF3D80AC001
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
Using BUSCO to Assess Insect Genomic Resources
Titre du livre
Methods in Molecular Biology
Auteur(s)
Waterhouse Robert M., Seppey Mathieu, Simão Felipe A., Zdobnov Evgeny M.
Editeur
Springer New York
Lieu d'édition
New York
ISBN
9781493987740
9781493987757
ISSN
1064-3745
1940-6029
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Pages
59-74
Langue
anglais
Résumé
The increasing affordability of sequencing technologies offers many new and exciting opportunities to address a diverse array of biological questions. This is evidenced in entomological research by numerous genomics and transcriptomics studies that attempt to decipher the often complex relationships amongst different species or orders and to build ‘omics’ resources to drive advancement of the molecular understanding of insect biology. Being able to gauge the quality of the sequencing data is of critical importance to understanding the potential limitations on the types of questions that these data can be reliably used to address. This chapter details the use of the Benchmarking Universal Single-Copy Orthologue (BUSCO) assessment tool to estimate the completeness of transcriptomes, genome assemblies, and annotated gene sets in terms of their expected gene content.
Mots-clé
Genomics, Transcriptomics, Genome annotation, Completeness assessment, Single - copy orthologues
Pubmed
Création de la notice
12/11/2018 11:16
Dernière modification de la notice
21/09/2019 18:38
Données d'usage