Glucocorticoids exert direct toxicity on microvasculature: analysis of cell death mechanisms.

Détails

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_EBC4BA09C322
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Glucocorticoids exert direct toxicity on microvasculature: analysis of cell death mechanisms.
Périodique
Toxicological Sciences
Auteur(s)
El Zaoui I., Behar-Cohen F., Torriglia A.
ISSN
1096-0929 (Electronic)
ISSN-L
1096-0929
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
143
Numéro
2
Pages
441-453
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Glucocorticoids (GCs) are routinely administered systemically or injected into the eye when treating numerous ocular diseases; however, their toxicity on the retinal microvasculature has not been previously investigated. In this article, the effects of hydrocortisone (Hydro), dexamethasone, dexamethasone-phosphate and triamcinolone acetonide (TA) were evaluated in vitro on human skin microcirculation cells and, bovine endothelial retinal cells, ex-vivo, on flat mounted rat retinas. The degree of GCs induced endothelial cell death varied according to the endothelial cell type and GCs chemical properties. GCs toxicity was higher in skin microvascular endothelial cells and for hydrophobic GC formulations. The mechanism of cell death differed between GCs, Hydro and TA activated the leukocyte elastase inhibitor/L-DNase II pathways but did not activate caspases. The mechanisms of cell death observed in cell cultures were similar to those observed in rat retinal explants. Taken together these results indicate that particular attention should be paid to the potential vascular side effects when administrating GCs clinically and in particular when developing sustained-release intraocular devices.
Pubmed
Web of science
Création de la notice
02/02/2015 15:26
Dernière modification de la notice
03/03/2018 22:28
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