Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.

Détails

ID Serval
serval:BIB_EBC2CE854B56
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.
Périodique
Molecular and Cellular Biology
Auteur(s)
Hu P., Wu S., Sun Y., Yuan C.C., Kobayashi R., Myers M.P., Hernandez N.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
2002
Volume
22
Numéro
22
Pages
8044-8055
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of similarity to the yeast subunits. We have purified an RNA polymerase III complex and identified its components. We found that two RNA polymerase III subunits, referred to as RPC8 and RPC9, displayed sequence similarity to the RNA polymerase II RPB7 and RPB4 subunits, respectively. RPC8 and RPC9 associated with each other, paralleling the association of the RNA polymerase II subunits, and were thus paralogues of RPB7 and RPB4. Furthermore, the complex contained a prominent 80-kDa polypeptide, which we called RPC5 and which corresponded to the human orthologue of the yeast C37 subunit despite limited sequence similarity. RPC5 associated with RPC53, the human orthologue of S. cerevisiae C53, paralleling the association of the S. cerevisiae C37 and C53 subunits, and was required for transcription from the type 2 VAI and type 3 human U6 promoters. Our results provide a characterization of human RNA polymerase III and show that the RPC5 subunit is essential for transcription.
Mots-clé
Amino Acid Sequence, Cloning, Molecular, Hela Cells, Humans, Macromolecular Substances, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Protein Subunits, RNA Polymerase III/chemistry, RNA Polymerase III/genetics, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 17:13
Données d'usage