Zwitterionic polysaccharides stimulate T cells with no preferential V beta usage and promote anergy, resulting in protection against experimental abscess formation.
Details
Serval ID
serval:BIB_EACF4D7E3C89
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Zwitterionic polysaccharides stimulate T cells with no preferential V beta usage and promote anergy, resulting in protection against experimental abscess formation.
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
2004
Volume
172
Number
3
Pages
1483-1490
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
Zwitterionic polysaccharides (Zps) from pathogenic bacteria, such as Bacteroides fragilis, are virulence factors responsible for abscess formation associated with intra-abdominal sepsis. The underlying cellular mechanism for abscess formation requires T cell activation. Conversely, abscess formation can be prevented by prophylactic s.c. injection of purified Zps alone, a process also dependent on T cells. Hence, the modulatory role of T cells in abscess formation was investigated. We show that Zps interact directly with T cells with fast association/dissociation kinetics. Vbeta repertoire analysis using RT-PCR demonstrates that Zps have broad Vbeta usage. Zps-specific hybridomas responded to a variety of other Zps, but not to a nonzwitterionic polysaccharide, indicating cross-reactivity between different Zps. Furthermore, Zps-reactive T cell hybridomas could effectively transfer protection against abscess formation. Analysis of the proliferative capacity of T cells recovered from Zps-treated animals revealed that these T cells are anergic to subsequent stimulation by the different Zps or to alloantigens in an MLR. This anergic response was relieved by addition of IL-2. Taken together, the data show that this class of polysaccharides interacts directly with T cells in a nonbiased manner to elicit an IL-2-dependent anergic response that confers protection against abscess formation.
Keywords
Abscess/immunology, Abscess/prevention & control, Animals, Antigens, Bacterial/immunology, Bacteroides Infections/immunology, Bacteroides Infections/microbiology, Bacteroides fragilis/immunology, Cell Communication/immunology, Cell Line, Tumor, Clonal Anergy/immunology, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/immunology, Hybridomas, Ions, Lymphocyte Activation/immunology, Male, Mice, Peptides/pharmacology, Pneumococcal Infections/immunology, Pneumococcal Infections/prevention & control, Polysaccharides, Bacterial/immunology, Polysaccharides, Bacterial/pharmacology, Rats, Rats, Inbred Lew/microbiology, Rats, Wistar, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, T-Lymphocytes/immunology, T-Lymphocytes/metabolism
Pubmed
Web of science
Create date
25/01/2008 15:53
Last modification date
20/08/2019 17:13