Rat PPARs: quantitative analysis in adult rat tissues and regulation in fasting and refeeding.

Details

Serval ID
serval:BIB_EAC91A2056B7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rat PPARs: quantitative analysis in adult rat tissues and regulation in fasting and refeeding.
Journal
Endocrinology
Author(s)
Escher P., Braissant O., Basu-Modak S., Michalik L., Wahli W., Desvergne B.
ISSN
0013-7227[print], 0013-7227[linking]
Publication state
Published
Issued date
10/2001
Volume
142
Number
10
Pages
4195-4202
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
PPARs are members of the nuclear hormone receptor superfamily and are primarily involved in lipid metabolism. The expression patterns of all 3 PPAR isotypes in 22 adult rat organs were analyzed by a quantitative ribonuclease protection assay. The data obtained allowed comparison of the expression of each isotype to the others and provided new insight into the less studied PPAR beta (NR1C2) expression and function. This isotype shows a ubiquitous expression pattern and is the most abundant of the three PPARs in all analyzed tissues except adipose tissue. Its expression is especially high in the digestive tract, in addition to kidney, heart, diaphragm, and esophagus. After an overnight fast, PPAR beta mRNA levels are dramatically down-regulated in liver and kidney by up to 80% and are rapidly restored to control levels upon refeeding. This tight nutritional regulation is independent of the circulating glucocorticoid levels and the presence of PPAR alpha, whose activity is markedly up-regulated in the liver and small intestine during fasting. Finally, PPAR gamma 2 mRNA levels are decreased by 50% during fasting in both white and brown adipose tissue. In conclusion, fasting can strongly influence PPAR expression, but in only a few selected tissues.
Keywords
Animals, DNA, Complementary/analysis, DNA, Complementary/genetics, Eating/physiology, Fasting/physiology, Gene Expression Regulation/physiology, Molecular Sequence Data, Nuclear Proteins/physiology, Organ Specificity/physiology, Rats, Receptors, Cytoplasmic and Nuclear/physiology, Signal Transduction, Transcription Factors/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:27
Last modification date
20/08/2019 16:13
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