TP53 alterations in pancreatic acinar cell carcinoma: new insights into the molecular pathology of this rare cancer.

Détails

ID Serval
serval:BIB_EA8A7ABDF8E6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
TP53 alterations in pancreatic acinar cell carcinoma: new insights into the molecular pathology of this rare cancer.
Périodique
Virchows Archiv : An International Journal of Pathology
Auteur(s)
La Rosa S., Bernasconi B., Frattini M., Tibiletti M.G., Molinari F., Furlan D., Sahnane N., Vanoli A., Albarello L., Zhang L., Notohara K., Casnedi S., Chenard M.P., Adsay V., Asioli S., Capella C., Sessa F.
ISSN
1432-2307 (Electronic)
ISSN-L
0945-6317
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
468
Numéro
3
Pages
289-296
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
The molecular alterations of pancreatic acinar cell carcinomas (ACCs) are poorly understood and have been reported as being different from those in ductal adenocarcinomas. Loss of TP53 gene function in the pathogenesis of ACCs is controversial since contradictory findings have been published. A comprehensive analysis of the different possible genetic and epigenetic mechanisms leading to TP53 alteration in ACC has never been reported and hence the role of TP53 in the pathogenesis and/or progression of ACC remains unclear. We investigated TP53 alterations in 54 tumor samples from 44 patients, including primary and metastatic ACC, using sequencing analysis, methylation-specific multiplex ligation probe amplification, fluorescence in situ hybridization, and immunohistochemistry. TP53 mutations were found in 13 % of primary ACCs and in 31 % of metastases. Primary ACCs and metastases showed the same mutational profile, with the exception of one case, characterized by a wild-type sequence in the primary carcinoma and a mutation in the corresponding metastasis. FISH analysis revealed deletion of the TP53 region in 53 % of primary ACCs and in 50 % of metastases. Promoter hypermethylation was found in one case. The molecular alterations correlated well with the immunohistochemical findings. A statistically significant association was found between the combination of mutation of one allele and loss of the other allele of TP53 and worse survival.
Mots-clé
Adult, Aged, Carcinoma, Acinar Cell/metabolism, Carcinoma, Acinar Cell/pathology, Female, Genes, p53/genetics, Humans, Immunohistochemistry/methods, In Situ Hybridization, Fluorescence/methods, Male, Middle Aged, Mutation/genetics, Pancreatic Neoplasms/genetics, Pancreatic Neoplasms/metabolism, Pathology, Molecular/methods, Promoter Regions, Genetic/genetics
Pubmed
Web of science
Création de la notice
06/09/2016 11:47
Dernière modification de la notice
20/08/2019 16:12
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