Simultaneous determination of selective serotonin reuptake inhibitors and their main metabolites in human breast milk by liquid chromatography-electrospray mass spectrometry.

Détails

ID Serval
serval:BIB_EA86621FAFDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Simultaneous determination of selective serotonin reuptake inhibitors and their main metabolites in human breast milk by liquid chromatography-electrospray mass spectrometry.
Périodique
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Auteur(s)
Weisskopf E., Panchaud A., Nguyen K.A., Grosjean D., Hascoët J.M., Csajka C., Eap C.B., Ansermot N.
Collaborateur(s)
collaborators of the SSRI-Breast Milk study
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Statut éditorial
Publié
Date de publication
01/07/2017
Peer-reviewed
Oui
Volume
1057
Pages
101-109
Langue
anglais
Notes
Publication types: Journal Article ; Validation Studies
Publication Status: ppublish
Résumé
A bioanalytical method by high performance liquid chromatography coupled to electrospray mass spectrometry (HPLC-ESI-MS), adapted from a previously published method in plasma, was validated in breast milk for the simultaneous quantification of all antidepressants belonging to the class of selective serotonin reuptake inhibitors (citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline) and their major metabolites (desmethylcitalopram and norfluoxetine). Milk samples (250μl) first underwent protein precipitation followed by solid-phase extraction on a reversed phase/cation exchange sorbent. Analytes were thereafter separated on a XBridge C18 column (2.1mm×100mm; 3.5μm) using a mobile phase composed of ammonium acetate buffer (pH 8.1; 50mM) and acetonitrile in gradient mode. Detection was performed by a single quadrupole mass spectrometer running in selected ion monitoring in positive ionization mode. Method validation covered a wide concentration range of 2-500ng/ml for citalopram, desmethylcitalopram and paroxetine, 5-500ng/ml for sertraline, and 2-1000ng/ml for fluoxetine, norfluoxetine and fluvoxamine. Validation performances such as trueness (90.3-111.6%), repeatability (0.8-9.3%) and intermediate precision (0.9-9.5%) were in agreement with criteria from international guidelines and matrix effects for the analyte/internal standard ratios ranged from 92% to 110% (relative standard deviation <15%). Accuracy profiles (total error of trueness and precision) were lying within the limits of ±30% accepted in bioanalysis. Finally, the method was successfully applied to patient samples collected in a clinical pharmacokinetic study of nursing mothers taking an antidepressant treatment.

Mots-clé
Calibration, Chromatography, High Pressure Liquid/methods, Female, Fluoxetine/analogs & derivatives, Fluoxetine/analysis, Humans, Metabolomics, Milk, Human/chemistry, Milk, Human/metabolism, Serotonin Uptake Inhibitors/administration & dosage, Serotonin Uptake Inhibitors/analysis, Serotonin Uptake Inhibitors/metabolism, Solid Phase Extraction, Tandem Mass Spectrometry/methods, Antidepressants, Breast milk, LC–MS, Quantification, SSRI
Pubmed
Web of science
Création de la notice
22/05/2017 8:55
Dernière modification de la notice
20/08/2019 16:12
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