Des antisérums dirigés contre l'antigène carcino-embryonnaire (CEA) peuvent induire une lyse spécifique des cellules de carcinome du côlon par des lymphocytes normaux

Details

Serval ID
serval:BIB_EA5FA04CB621
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Des antisérums dirigés contre l'antigène carcino-embryonnaire (CEA) peuvent induire une lyse spécifique des cellules de carcinome du côlon par des lymphocytes normaux
Journal
Schweizerische Medizinische Wochenschrift
Author(s)
Carrel S.t., Delisle M.C., Mach J.P.
ISSN
0036-7672 (Print)
ISSN-L
0036-7672
Publication state
Published
Issued date
1978
Volume
108
Number
25
Pages
954-958
Language
french
Notes
[Antiserums against carcino-embryonic antigen (CEA) can induce a specific lysis of colon carcinoma cells by normal lymphocytes].
Abstract
Antibody-dependent cell-mediated cytotoxicity (ADCC) against human colon carcinoma cells grown in vitro was demonstrated with two specific rabbit anti-carcinoembryonic antigen (cea) antisera. The same antisera did not lyse the colon carcinoma cells in the presence of complement but without lymphocytes. The normal human lymphocytes in the absence of anti-CEA antiserum had a very low cytotoxic activity during the three hours 51Cr release assay used in this study. Two colon carcinoma cell lines, HT-29 and Co-115, expressing CEA on their surface as demonstrated by immunofluorescence, were significantly lysed in the ADCC test, whereas control tumor cell lines, not expressing CEA, were not affected by the anti-CEA sera and the lymphocytes. The specificity of the reaction was further demonstrated by the inhibition of antibody-dependent cell-mediated cytotoxicity after the addition of increasing amounts of purified CEA to the antiserum. The absorption of the anti-CEA antisera was controlled by radioimmunoassay. Absorption of the antisera by normal lung extracts and red cells of different blood groups did not decrease the cytotoxicity.
Keywords
Animals, Antibody-Dependent Cell Cytotoxicity, Carcinoembryonic Antigen, Cell Line, Colonic Neoplasms/immunology, Humans, Immune Sera, Lymphocytes/immunology, Rabbits/immunology
Pubmed
Web of science
Create date
25/04/2013 10:20
Last modification date
20/08/2019 16:12
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