Article: article from journal or magazin.
Ectopic lymphoid-organ development occurs through interleukin 7-mediated enhanced survival of lymphoid-tissue-inducer cells.
Development of Peyer's patches and lymph nodes requires the interaction between CD4+ CD3- IL-7Ralpha+ lymphoid-tissue inducer (LTi) and VCAM-1+ organizer cells. Here we showed that by promoting their survival, enhanced expression of interleukin-7 (IL-7) in transgenic mice resulted in accumulation of LTi cells. With increased IL-7 availability, de novo formation of VCAM-1+ Peyer's patch anlagen occurred along the entire fetal gut resulting in a 5-fold increase in Peyer's patch numbers. IL-7 overexpression also led to formation of multiple organized ectopic lymph nodes and cecal patches. After immunization, ectopic lymph nodes developed normal T cell-dependent B cell responses and germinal centers. Mice overexpressing IL-7 but lacking either RORgamma, a factor required for LTi cell generation, or lymphotoxin alpha1beta2 had neither Peyer's patches nor ectopic lymph nodes. Therefore, by controlling LTi cell numbers, IL-7 can regulate the formation of both normal and ectopic lymphoid organs.
Animals, Cell Differentiation/immunology, Cell Survival, Cells, Cultured, Fetus/metabolism, Gene Expression Regulation, Interleukin-7/genetics, Interleukin-7/metabolism, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Mice, Mice, Transgenic, Stem Cells/cytology, Stem Cells/metabolism, T-Lymphocytes, Helper-Inducer/cytology, T-Lymphocytes, Helper-Inducer/immunology, Vascular Cell Adhesion Molecule-1/metabolism
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