The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replication in vivo.

Détails

ID Serval
serval:BIB_EA36FD9A7818
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replication in vivo.
Périodique
Journal of Biological Chemistry
Auteur(s)
Müller K., Mermod N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2000
Volume
275
Numéro
3
Pages
1645-1650
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Efficient initiation of SV40 DNA replication requires transcription factors that bind auxiliary sequences flanking the minimally required origin. To evaluate the possibility that transcription factors may activate SV40 replication by acting on the chromatin structure of the origin, we used an in vivo replication system in which we targeted GAL4 fusion proteins to the minimally required origin. We found that the proline-rich transcriptional activation domain of nuclear factor I (NF-I), which has been previously shown to interact with histone H3, specifically activates replication. Evaluation of a series of deletion and point mutants of NF-I indicates that the H3-binding domain and the replication activity coincide perfectly. Assays with other transcription factors, such as Sp1, confirmed the correlation between the interaction with H3 and the activation of replication. These findings imply that transcription factors such as NF-I can activate SV40 replication via direct interaction with chromatin components, thereby contributing to the relief of nucleosomal repression at the SV40 origin.
Mots-clé
Animals, CCAAT-Enhancer-Binding Proteins, COS Cells, Carrier Proteins/metabolism, Chromatin/metabolism, DNA Replication, DNA, Viral/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Histones/metabolism, NFI Transcription Factors, Organic Cation Transport Proteins, Plasmids/metabolism, Point Mutation, Protein Structure, Tertiary, Simian virus 40/genetics, Simian virus 40/metabolism, Sp1 Transcription Factor/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcriptional Activation, Y-Box-Binding Protein 1
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 10:41
Dernière modification de la notice
20/08/2019 16:12
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