Ubiquitination of HEXIM1 by HDM2.
Details
Serval ID
serval:BIB_E9EA4E0DE384
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ubiquitination of HEXIM1 by HDM2.
Journal
Cell Cycle
ISSN
1551-4005[electronic], 1551-4005[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
8
Number
14
Pages
2247-2254
Language
english
Abstract
Hexamethylene bis-acetamide inducible protein 1 (HEXIM1) is an inhibitor of the positive transcription elongation factor b (P-TEFb), which controls RNA polymerase II transcription and human immunodeficiency virus Tat transactivation. In cells, more than half of P-TEFb is associated with HEXIM1 resulting in the inactivation of P-TEFb. Recently, we found that nucleophosmin (NPM), a key factor involved in p53 signaling pathway, interacts with HEXIM1 and activates P-TEFb-dependent transcription. Here we report that human double minute-2 protein (HDM2), a p53-specific E3 ubiquitin ligase, specifically ubiquitinates HEXIM1 through the lysine residues located within the basic region of HEXIM1. However, the HDM2-induced HEXIM1 ubiquitination does not lead to proteasome-mediated protein degradation. Fusion of ubiquitin to HEXIM1 demonstrates stronger inhibition on P-TEFb-dependent transcription. Our results demonstrate that HDM2 functions as a specific E3 ubiquitin ligase for HEXIM1, suggesting a possible role for HEXIM1 ubiquitination in the regulation of P-TEFb activity.
Keywords
Cell Line, Cysteine Proteinase Inhibitors/pharmacology, Humans, Leupeptins/pharmacology, Nuclear Proteins/metabolism, Positive Transcriptional Elongation Factor B/genetics, Positive Transcriptional Elongation Factor B/metabolism, Proto-Oncogene Proteins c-mdm2/metabolism, RNA-Binding Proteins/metabolism, Signal Transduction, Tumor Suppressor Protein p53/metabolism, Ubiquitin-Protein Ligases/metabolism, Ubiquitination
Pubmed
Web of science
Create date
15/05/2009 16:15
Last modification date
20/08/2019 17:12