Article: article from journal or magazin.
Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression.
Journal of Biological Chemistry
The SNARE proteins are essential components of the intracellular fusion machinery. It is thought that they form a tight four-helix complex between membranes, in effect initiating fusion. Most SNAREs contain a single coiled-coil region, referred to as the SNARE motif, directly adjacent to a single transmembrane domain. The neuronal SNARE SNAP-25 defines a subfamily of SNARE proteins with two SNARE helices connected by a longer linker, comprising also the proteins SNAP-23 and SNAP-29. We now report the initial characterization of a novel vertebrate homologue termed SNAP-47. Northern blot and immunoblot analysis revealed ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP-47 shows a widespread distribution on intracellular membranes and is also enriched in synaptic vesicle fractions. In vitro, SNAP-47 substituted for SNAP-25 in SNARE complex formation with the neuronal SNAREs syntaxin 1a and synaptobrevin 2, and it also substituted for SNAP-25 in proteoliposome fusion. However, neither complex assembly nor fusion was as efficient as with SNAP-25.
Amino Acid Motifs, Amino Acid Sequence, Animals, Gene Expression Regulation, Humans, Molecular Sequence Data, Neurons/metabolism, Qb-SNARE Proteins/biosynthesis, Qb-SNARE Proteins/chemistry, Qc-SNARE Proteins/biosynthesis, Qc-SNARE Proteins/chemistry, Rats, SNARE Proteins/biosynthesis, SNARE Proteins/physiology, Synaptosomal-Associated Protein 25/biosynthesis, Tissue Distribution, Vesicular Transport Proteins/biosynthesis
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