Article: article from journal or magazin.
The complementary strand of the human T-cell leukemia virus type 1 RNA genome encodes a bZIP transcription factor that down-regulates viral transcription.
Journal of Virology
The RNA genome of the human T-cell leukemia virus type 1 (HTLV-1) codes for proteins involved in infectivity, replication, and transformation. We report in this study the characterization of a novel viral protein encoded by the complementary strand of the HTLV-1 RNA genome. This protein, designated HBZ (for HTLV-1 bZIP factor), contains a N-terminal transcriptional activation domain and a leucine zipper motif in its C terminus. We show here that HBZ is able to interact with the bZIP transcription factor CREB-2 (also called ATF-4), known to activate the HTLV-1 transcription by recruiting the viral trans-activator Tax on the Tax-responsive elements (TxREs). However, we demonstrate that the HBZ/CREB-2 heterodimers are no more able to bind to the TxRE and cyclic AMP response element sites. Taking these findings together, the functional inactivation of CREB-2 by HBZ is suggested to contribute to regulation of the HTLV-1 transcription. Moreover, the characterization of a minus-strand gene protein encoded by HTLV-1 has never been reported until now.
Activating Transcription Factor 4, Amino Acid Sequence, Animals, Basic-Leucine Zipper Transcription Factors, COS Cells, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Down-Regulation, G-Box Binding Factors, Gene Expression Regulation, Viral, Human T-lymphotropic virus 1/genetics, Molecular Sequence Data, RNA, Complementary/genetics, RNA, Viral/genetics, Transcription Factors/chemistry, Transcription Factors/genetics, Transcription, Genetic
Web of science
Last modification date