Flexible DNA binding of the BTB/POZ-domain protein FBI-1.

Détails

ID Serval
serval:BIB_E95FF436DF92
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Flexible DNA binding of the BTB/POZ-domain protein FBI-1.
Périodique
Journal of Biological Chemistry
Auteur(s)
Pessler F., Hernandez N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
278
Numéro
31
Pages
29327-29335
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
POZ-domain transcription factors are characterized by the presence of a protein-protein interaction domain called the POZ or BTB domain at their N terminus and zinc fingers at their C terminus. Despite the large number of POZ-domain transcription factors that have been identified to date and the significant insights that have been gained into their cellular functions, relatively little is known about their DNA binding properties. FBI-1 is a BTB/POZ-domain protein that has been shown to modulate HIV-1 Tat trans-activation and to repress transcription of some cellular genes. We have used various viral and cellular FBI-1 binding sites to characterize the interaction of a POZ-domain protein with DNA in detail. We find that FBI-1 binds to inverted sequence repeats downstream of the HIV-1 transcription start site. Remarkably, it binds efficiently to probes carrying these repeats in various orientations and spacings with no particular rotational alignment, indicating that its interaction with DNA is highly flexible. Indeed, FBI-1 binding sites in the adenovirus 2 major late promoter, the c-fos gene, and the c-myc P1 and P2 promoters reveal variously spaced direct, inverted, and everted sequence repeats with the consensus sequence G(A/G)GGG(T/C)(C/T)(T/C)(C/T) for each repeat.
Mots-clé
Adenoviridae/genetics, Base Sequence, Binding Sites, Consensus Sequence, DNA Methylation, DNA, Viral/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Genes, fos/genetics, Genes, myc/genetics, HIV-1/genetics, Humans, Molecular Sequence Data, Mutagenesis, Site-Directed, Promoter Regions, Genetic/genetics, Repetitive Sequences, Nucleic Acid, Transcription Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
20/08/2019 17:12
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