Multicenter analysis of sputum microbiota in tuberculosis patients.

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State: Public
Version: Final published version
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Serval ID
serval:BIB_E924DB344DCF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Multicenter analysis of sputum microbiota in tuberculosis patients.
Journal
PloS one
Author(s)
Sala C., Benjak A., Goletti D., Banu S., Mazza-Stalder Jesica, Jaton K., Busso P., Remm S., Leleu M., Rougemont J., Palmieri F., Cuzzi G., Butera O., Vanini V., Kabir S., Rahman SMM, Nicod L., Cole S.T.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
15
Number
10
Pages
e0240250
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The impact of tuberculosis and of anti-tuberculosis therapy on composition and modification of human lung microbiota has been the object of several investigations. However, no clear outcome has been presented so far and the relationship between M. tuberculosis pulmonary infection and the resident lung microbiota remains vague. In this work we describe the results obtained from a multicenter study of the microbiota of sputum samples from patients with tuberculosis or unrelated lung diseases and healthy donors recruited in Switzerland, Italy and Bangladesh, with the ultimate goal of discovering a microbiota-based biomarker associated with tuberculosis. Bacterial 16S rDNA amplification, high-throughput sequencing and extensive bioinformatic analyses revealed patient-specific flora and high variability in taxon abundance. No common signature could be identified among the individuals enrolled except for minor differences which were not consistent among the different geographical settings. Moreover, anti-tuberculosis therapy did not cause any important variation in microbiota diversity, thus precluding its exploitation as a biomarker for the follow up of tuberculosis patients undergoing treatment.
Pubmed
Web of science
Open Access
Yes
Create date
19/10/2020 14:45
Last modification date
30/04/2021 6:15
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