Heptageniidae is a species-rich mayfly family (Ephemeroptera), whose taxonomy and phylogeny have been based almost exclusively on traditional morphological studies. Inconsistent use of diagnostic characters and the general lack of molecular studies have led to vague generic concepts, and the phylogenetic relationships among taxa in the family remain unclear. AfronurusLestage is an Old World heptageniid genus of 66 species. The generic assignment of two species within this genus, A.kugleriDemoulin and A.zebratus (Hagen), has been the subject of much debate, because they share many apomorphic features that distinguish them from other congeners. We combined a thorough morphological study of all life stages of 28 representative heptageniid species with a molecular phylogenetic analysis of four mitochondrial and nuclear markers to resolve the generic position of A.kugleri and A.zebratus as well as the integrity of Afronurus and related genera. Our results confirm the monophyly of Afronurus and Electrogena and support the assignment of A.kugleri and A.zebratus to a newly described genus, AnaposYanai & Sartori gen.n. We provide clear, diagnostic morphological characters for the genus and discuss the need for a thorough revision of generic concepts in the subfamily Ecdyonurinae. This published work has been registered in ZooBank, .