Ezrin directly interacts with AQP2 and promotes its endocytosis.

Détails

ID Serval
serval:BIB_E8518FC941FB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ezrin directly interacts with AQP2 and promotes its endocytosis.
Périodique
Journal of cell science
Auteur(s)
Li W., Jin W.W., Tsuji K., Chen Y., Nomura N., Su L., Yui N., Arthur J., Cotecchia S., Paunescu T.G., Brown D., Lu HAJ
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Statut éditorial
Publié
Date de publication
01/09/2017
Peer-reviewed
Oui
Volume
130
Numéro
17
Pages
2914-2925
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The water channel aquaporin-2 (AQP2) is a major regulator of water homeostasis in response to vasopressin (VP). Dynamic trafficking of AQP2 relies on its close interaction with trafficking machinery proteins and the actin cytoskeleton. Here, we report the identification of ezrin, an actin-binding protein from the ezrin/radixin/moesin (ERM) family as an AQP2-interacting protein. Ezrin was first detected in a co-immunoprecipitation (co-IP) complex using an anti-AQP2 antibody in a proteomic analysis. Immunofluorescence staining revealed the co-expression of ezrin and AQP2 in collecting duct principal cells, and VP treatment caused redistribution of both proteins to the apical membrane. The ezrin-AQP2 interaction was confirmed by co-IP experiments with an anti-ezrin antibody, and by pulldown assays using purified full-length and FERM domain-containing recombinant ezrin. By using purified recombinant proteins, we showed that ezrin directly interacts with AQP2 C-terminus through its N-terminal FERM domain. Knocking down ezrin expression with shRNA resulted in increased membrane accumulation of AQP2 and reduced AQP2 endocytosis. Therefore, through direct interaction with AQP2, ezrin facilitates AQP2 endocytosis, thus linking the dynamic actin cytoskeleton network with AQP2 trafficking.

Mots-clé
Animals, Aquaporin 2/metabolism, Cell Membrane/metabolism, Clathrin/metabolism, Cyclic AMP/metabolism, Cytoskeletal Proteins/chemistry, Cytoskeletal Proteins/metabolism, Dogs, Down-Regulation, Endocytosis, Exocytosis, Gene Knockdown Techniques, Humans, Immunoprecipitation, LLC-PK1 Cells, Madin Darby Canine Kidney Cells, Phosphorylation, Protein Binding, Protein Domains, Rats, Swine, Vasopressins, Aquaporin-2, Ezrin, Trafficking, Water homeostasis
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/08/2017 11:43
Dernière modification de la notice
20/08/2019 16:11
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