Article: article from journal or magazin.
Detecting natural abundance carbon signal of NAA metabolite within 12-cm3 localized volume of human brain using 1H-[13C] NMR spectroscopy.
Magnetic Resonance in Medicine
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
NMR spectroscopy has been applied extensively to study metabolism noninvasively in the human brain and other tissues. However, it usually suffers from poor signal-to-noise ratio due to low NMR sensitivity and low metabolite concentrations. In this study, the technique of proton-observe-carbon-edited (POCE) NMR spectroscopy combined with a single-shot localization sequence was used to detect the natural abundance carbon signal of the amino acid N-acetyl aspartate from a 12-cm3 localized volume in the occipital lobe of humans at 4 T. The results suggest that NMR spectroscopy is sensitive enough to detect signals from low concentration metabolites (< 60 nmol/g) from small volumes in the human brain within several minutes of data acquisition. This reveals that in vivo NMR spectroscopy is a promising technique for detecting small metabolite changes and low traces of 13C isotopic labeling for dynamic metabolism studies aimed at investigating physiological and pathological questions.
Aspartic Acid/analogs & derivatives, Aspartic Acid/metabolism, Brain/metabolism, Carbon/metabolism, Humans, Magnetic Resonance Spectroscopy/instrumentation, Sensitivity and Specificity, Signal Processing, Computer-Assisted/instrumentation
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