Detecting natural abundance carbon signal of NAA metabolite within 12-cm3 localized volume of human brain using 1H-[13C] NMR spectroscopy.

Détails

ID Serval
serval:BIB_E7C70A2D7E01
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detecting natural abundance carbon signal of NAA metabolite within 12-cm3 localized volume of human brain using 1H-[13C] NMR spectroscopy.
Périodique
Magnetic Resonance in Medicine
Auteur(s)
Chen W., Adriany G., Zhu X.H., Gruetter R., Ugurbil K.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
1998
Volume
40
Numéro
2
Pages
180-184
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
NMR spectroscopy has been applied extensively to study metabolism noninvasively in the human brain and other tissues. However, it usually suffers from poor signal-to-noise ratio due to low NMR sensitivity and low metabolite concentrations. In this study, the technique of proton-observe-carbon-edited (POCE) NMR spectroscopy combined with a single-shot localization sequence was used to detect the natural abundance carbon signal of the amino acid N-acetyl aspartate from a 12-cm3 localized volume in the occipital lobe of humans at 4 T. The results suggest that NMR spectroscopy is sensitive enough to detect signals from low concentration metabolites (< 60 nmol/g) from small volumes in the human brain within several minutes of data acquisition. This reveals that in vivo NMR spectroscopy is a promising technique for detecting small metabolite changes and low traces of 13C isotopic labeling for dynamic metabolism studies aimed at investigating physiological and pathological questions.
Mots-clé
Aspartic Acid/analogs & derivatives, Aspartic Acid/metabolism, Brain/metabolism, Carbon/metabolism, Humans, Magnetic Resonance Spectroscopy/instrumentation, Sensitivity and Specificity, Signal Processing, Computer-Assisted/instrumentation
Pubmed
Web of science
Création de la notice
04/08/2010 16:28
Dernière modification de la notice
03/03/2018 22:20
Données d'usage