A shared surface of TBP directs RNA polymerase II and III transcription via association with different TFIIB family members.

Détails

ID Serval
serval:BIB_E75F0F9CC535
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A shared surface of TBP directs RNA polymerase II and III transcription via association with different TFIIB family members.
Périodique
Molecular Cell
Auteur(s)
Zhao X., Schramm L., Hernandez N., Herr W.
ISSN
1097-2765[print], 1097-2765[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
11
Numéro
1
Pages
151-161
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The TATA box binding protein TBP is highly conserved and the only known basal factor that is involved in transcription by all three eukaryotic nuclear RNA polymerases from promoters with or without a TATA box. By mutagenesis and analysis on a selected set of four model pol II and pol III TATA box-containing and TATA-less promoters, we demonstrate that human TBP utilizes two modes to achieve its versatile functions. First, it uses a different set of surfaces on the conserved and structured TBP core domain to direct transcription from each of the four model promoters. Second, unlike yeast TBP, human TBP can use a shared surface to interact with two different TFIIB family members--TFIIB and Brf2--to initiate transcription by different RNA polymerases.
Mots-clé
DNA/chemistry, DNA/metabolism, Hela Cells, Humans, Models, Molecular, Multigene Family, Mutagenesis, Site-Directed, Nucleic Acid Conformation, Promoter Regions, Genetic, Protein Binding, Protein Structure, Tertiary, RNA Polymerase II/genetics, RNA Polymerase II/metabolism, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Saccharomyces cerevisiae Proteins, TATA-Binding Protein Associated Factors, TATA-Box Binding Protein/chemistry, TATA-Box Binding Protein/genetics, Transcription Factor TFIIB/genetics, Transcription Factor TFIIB/metabolism, Transcription Factor TFIIIB/genetics, Transcription Factor TFIIIB/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 16:10
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