The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection.

Détails

ID Serval
serval:BIB_E750637DDD09
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection.
Périodique
Virology
Auteur(s)
Plattet P., Rivals J.P., Zuber B., Brunner J.M., Zurbriggen A., Wittek R.
ISSN
0042-6822[print], 0042-6822[linking]
Statut éditorial
Publié
Date de publication
07/2005
Volume
337
Numéro
2
Pages
312-326
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The wild-type A75/17 canine distemper virus (CDV) strain induces a persistent infection in the central nervous system but infects cell lines very inefficiently. In contrast, the genetically more distant Onderstepoort CDV vaccine strain (OP-CDV) induces extensive syncytia formation. Here, we investigated the roles of wild-type fusion (F(WT)) and attachment (H(WT)) proteins in Vero cells expressing, or not, the canine SLAM receptor by transfection experiments and by studying recombinants viruses expressing different combinations of wild-type and OP-CDV glycoproteins. We show that low fusogenicity is not due to a defect of the envelope proteins to reach the cell surface and that H(WT) determines persistent infection in a receptor-dependent manner, emphasizing the role of SLAM as a potent enhancer of fusogenicity. However, importantly, F(WT) reduced cell-to-cell fusion independently of the cell surface receptor, thus demonstrating that the fusion protein of the neurovirulent A75/17-CDV strain plays a key role in determining persistent infection.
Mots-clé
Amino Acids/analysis, Animals, Biotinylation, Cercopithecus aethiops, Distemper/virology, Distemper Virus, Canine/pathogenicity, Dogs, Fluorescent Antibody Technique, Indirect, Transfection, Vero Cells, Viral Fusion Proteins/analysis, Viral Fusion Proteins/chemistry
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 11:43
Dernière modification de la notice
09/05/2019 2:45
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