Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts.

Détails

ID Serval
serval:BIB_E72E7BFF03E0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts.
Périodique
Molecular and Cellular Biology
Auteur(s)
Pessler F., Pendergrast P.S., Hernandez N.
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Statut éditorial
Publié
Date de publication
07/1997
Volume
17
Numéro
7
Pages
3786-3798
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human immunodeficiency virus (HIV-1) promoter directs the synthesis of two classes of RNA molecules, short transcripts and full-length transcripts. The synthesis of short transcripts depends on a bipartite DNA element, the inducer of short transcripts (IST), located in large part downstream of the HIV-1 start site of transcription. IST does not require any viral product for function and is thought to direct the assembly of transcription complexes that are incapable of efficient elongation. Nothing is known, however, about the biochemical mechanisms that mediate IST function. Here, we report the identification and purification of a factor that binds specifically to the IST. This factor, FBI-1, recognizes a large bipartite binding site that coincides with the bipartite IST element. It is constituted at least in part by an 86-kDa polypeptide that can be specifically cross-linked to IST. FBI-1 also binds to promoter and attenuation regions of a number of cellular and viral transcription units that are regulated by a transcription elongation block. This observation, together with the observation that the binding of FBI-1 to IST mutants correlates with the ability of these mutants to direct IST function, suggests that FBI-1 may be involved in the establishment of abortive transcription complexes.
Mots-clé
Base Sequence, Binding Sites, DNA-Binding Proteins/isolation & purification, DNA-Binding Proteins/metabolism, Gene Expression Regulation, Gene Expression Regulation, Viral, HIV-1/genetics, HeLa Cells, Humans, Molecular Sequence Data, Molecular Weight, Promoter Regions, Genetic, RNA, Viral/genetics, Regulatory Sequences, Nucleic Acid, Transcription Factors/isolation & purification, Transcription Factors/physiology, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 17:10
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