Hyperglycaemia is inversely correlated with live M. bovis BCG-specific CD4<sup>+</sup> T cell responses in Tanzanian adults with latent or active tuberculosis.

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Serval ID
serval:BIB_E678D6B1F88E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hyperglycaemia is inversely correlated with live M. bovis BCG-specific CD4<sup>+</sup> T cell responses in Tanzanian adults with latent or active tuberculosis.
Journal
Immunity, inflammation and disease
Author(s)
Boillat-Blanco N., Tumbo A.N., Perreau M., Amelio P., Ramaiya K.L., Mganga M., Schindler C., Gagneux S., Reither K., Probst-Hensch N., Pantaleo G., Daubenberger C., Portevin D.
ISSN
2050-4527 (Electronic)
ISSN-L
2050-4527
Publication state
Published
Issued date
06/2018
Peer-reviewed
Oui
Volume
6
Number
2
Pages
345-353
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The rising prevalence of Diabetes mellitus (DM) in high TB-endemic countries may adversely affect sustainability of TB control since DM constitutes a risk factor for development of active tuberculosis (TB). The impact of DM on TB specific adaptive immune responses remains poorly addressed, particularly in people living in Sub-Saharan countries. We performed a functional characterization of TB specific cellular immune response in Tanzanian subjects with active or latent Mycobacterium tuberculosis (Mtb) infection stratified by their diabetic status.
HIV negative active TB patients (≥18 years) with Xpert MTB/RIF positive pulmonary TB were included before starting TB treatment in Dar es Salaam, Tanzania between April and December 2013. HIV negative healthy controls latently infected with TB but without past TB history were also included. Active and latent TB patients were stratified in two groups according to their diabetic status. Peripheral Blood Mononuclear cells were stimulated with either live M. bovis BCG or Mtb-specific peptide pools and analyzed by intracellular cytokine staining and polychromatic flow cytometry.
Our results show a lower frequency of IFN-γ CD4 <sup>+</sup> T cells in patients with active TB and DM compared to patients with active TB only after live M. bovis BCG (p = 0.04) but not after Mtb peptide pools re-stimulation. Irrespective of TB status, level of glycaemia is selectively inversely correlated with IFN-γ and TNF-α CD4 <sup>+</sup> T cell production (p = 0.02 and p = 0.03) after live M. bovis BCG stimulation.
These results support the hypothesis that hyperglycaemia negatively impacts antigen processing and/or presentation of whole mycobacteria delaying secretion of key cytokines involved in TB immunity.
Keywords
Adolescent, Adult, Antigens, Bacterial/immunology, Blood Glucose/immunology, Case-Control Studies, Comorbidity, Diabetes Mellitus/blood, Diabetes Mellitus/epidemiology, Diabetes Mellitus/immunology, Disease Progression, Female, Flow Cytometry, Humans, Hyperglycemia/blood, Hyperglycemia/epidemiology, Hyperglycemia/immunology, Latent Tuberculosis/epidemiology, Latent Tuberculosis/immunology, Latent Tuberculosis/microbiology, Lymphocyte Activation, Male, Middle Aged, Mycobacterium bovis/immunology, Mycobacterium tuberculosis/immunology, Mycobacterium tuberculosis/isolation & purification, Prospective Studies, T-Lymphocytes/immunology, Tanzania/epidemiology, Tuberculosis, Pulmonary/epidemiology, Tuberculosis, Pulmonary/immunology, Young Adult, Adaptive immunity, diabetes, hyperglycaemia, tuberculosis
Pubmed
Web of science
Open Access
Yes
Create date
14/04/2018 8:39
Last modification date
20/08/2019 16:09
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