Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba.

Détails

ID Serval
serval:BIB_E598596992A2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba.
Périodique
Optics Express
Auteur(s)
Charrière F., Pavillon N., Colomb T., Depeursinge C., Heger T.J., Mitchell E.A., Marquet P., Rappaz B.
ISSN
1094-4087[electronic]
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
14
Numéro
16
Pages
7005-7013
Langue
anglais
Résumé
This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of pi, allowing to built 3-dimensional quantitative refractive index distributions by an inverse Radon transform. A 20x magnification allows a resolution better than 3 microm in all three dimensions, with accuracy better than 0.01 for the refractive index measurements. This technique is for the first time to our knowledge applied to living specimen (testate amoeba, Protista). Morphometric measurements are extracted from the tomographic reconstructions, showing that the commonly used method for testate amoeba biovolume evaluation leads to systematic under evaluations by about 50%.
Mots-clé
Amoeba/ultrastructure, Animals, Holography/methods, Image Enhancement/methods, Microscopy/methods, Refractometry/methods, Signal Processing, Computer-Assisted, Tomography, Optical/methods
Pubmed
Web of science
Création de la notice
10/03/2008 16:40
Dernière modification de la notice
20/08/2019 16:08
Données d'usage