Article: article from journal or magazin.
Maintenance and polarization of human TH2 central memory T cells by thymic stromal lymphopoietin-activated dendritic cells.
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
The identity of TH2 memory cells and the mechanism regulating their maintenance during allergic inflammation remain elusive. We report that circulated human CD4+ T cells expressing the prostaglandin D2 receptor (CRTH2) are TH2 central memory T cells, characterized by their phenotype, TH2 cytokine production, gene-expression profile, and the ability to respond to allergens. Only dendritic cells (DCs) activated by thymic stromal lymphopoietin (TSLP) can induce a robust expansion of CRTH2+CD4+ TH2 memory cells, while maintaining their central memory phenotype and TH2 commitments. CRTH2+CD4+ TH2 memory cells activated by TSLP-DCs undergo further TH2 polarization and express cystatin A, Charcot-Leydon crystal protein, and prostaglandin D2 synthase, implying their broader roles in allergic inflammation. Infiltrated CRTH2+CD4+ TH2 effector memory T cells in skin lesion of atopic dermatitis were associated with activated DCs, suggesting that TSLP-DCs play important roles not only in TH2 priming, but also in the maintenance and further polarization of TH2 central memory cells in allergic diseases.
Antigens, Differentiation/metabolism, Cell Polarity/genetics, Cells, Cultured, Cyclin-Dependent Kinase Inhibitor Proteins/genetics, Cyclin-Dependent Kinase Inhibitor Proteins/metabolism, Cystatins/genetics, Cytokines/genetics, Cytokines/pharmacology, Dendritic Cells/drug effects, Dendritic Cells/immunology, Dermatitis, Atopic/genetics, Dermatitis, Atopic/immunology, Gene Expression Profiling, Glycoproteins/genetics, Humans, Immunologic Memory/genetics, Intramolecular Oxidoreductases/genetics, Lipocalins, Lymphocyte Activation/genetics, Lysophospholipase/genetics, Membrane Glycoproteins/metabolism, OX40 Ligand, Receptors, Immunologic/analysis, Receptors, Immunologic/metabolism, Receptors, Prostaglandin/analysis, Receptors, Prostaglandin/metabolism, Th2 Cells/immunology, Tumor Necrosis Factors/metabolism
Web of science
Last modification date