Semi-supervised integration of single-cell transcriptomics data.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_E4C01949C6C2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Semi-supervised integration of single-cell transcriptomics data.
Journal
Nature communications
Author(s)
Andreatta M., Hérault L., Gueguen P., Gfeller D., Berenstein A.J., Carmona S.J.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
29/01/2024
Peer-reviewed
Oui
Volume
15
Number
1
Pages
872
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Batch effects in single-cell RNA-seq data pose a significant challenge for comparative analyses across samples, individuals, and conditions. Although batch effect correction methods are routinely applied, data integration often leads to overcorrection and can result in the loss of biological variability. In this work we present STACAS, a batch correction method for scRNA-seq that leverages prior knowledge on cell types to preserve biological variability upon integration. Through an open-source benchmark, we show that semi-supervised STACAS outperforms state-of-the-art unsupervised methods, as well as supervised methods such as scANVI and scGen. STACAS scales well to large datasets and is robust to incomplete and imprecise input cell type labels, which are commonly encountered in real-life integration tasks. We argue that the incorporation of prior cell type information should be a common practice in single-cell data integration, and we provide a flexible framework for semi-supervised batch effect correction.
Keywords
Humans, Sequence Analysis, RNA/methods, Single-Cell Analysis/methods, Gene Expression Profiling/methods
Pubmed
Web of science
Open Access
Yes
Create date
01/02/2024 15:32
Last modification date
09/08/2024 15:07
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