Article: article from journal or magazin.
Transient activation of the HOG MAPK pathway regulates bimodal gene expression.
Mitogen-activated protein kinase (MAPK) cascades are conserved signaling modules that control many cellular processes by integrating intra- and extracellular cues. The p38/Hog1 MAPK is transiently activated in response to osmotic stress, leading to rapid translocation into the nucleus and induction of a specific transcriptional program. When investigating the dynamic interplay between Hog1 activation and Hog1-driven gene expression, we found that Hog1 activation increases linearly with stimulus, whereas the transcriptional output is bimodal. Modeling predictions, corroborated by single-cell experiments, established that a slow stochastic transition from a repressed to an activated transcriptional state in conjunction with transient Hog1 activation generates this behavior. Together, these findings provide a molecular mechanism by which a cell can impose a transcriptional threshold in response to a linear signaling behavior.
Cell Nucleus/metabolism, Chromatin Assembly and Disassembly, Enzyme Activation, Gene Expression Regulation, Fungal, Genes, Fungal, MAP Kinase Signaling System, Mitogen-Activated Protein Kinases/genetics, Mitogen-Activated Protein Kinases/metabolism, Models, Genetic, Osmolar Concentration, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Stochastic Processes, Stress, Physiological, Transcriptional Activation
Web of science
Last modification date