Fibroblastic reticular cells from lymph nodes attenuate T cell expansion by producing nitric oxide.

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Serval ID
serval:BIB_E37A5F5E8F2F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fibroblastic reticular cells from lymph nodes attenuate T cell expansion by producing nitric oxide.
Journal
PLoS One
Author(s)
Siegert S., Huang H.Y., Yang C.Y., Scarpellino L., Carrie L., Essex S., Nelson P.J., Heikenwalder M., Acha-Orbea H., Buckley C.D., Marsland B.J., Zehn D., Luther S.A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2011
Volume
6
Number
11
Pages
e27618
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Adaptive immune responses are initiated when T cells encounter antigen on dendritic cells (DC) in T zones of secondary lymphoid organs. T zones contain a 3-dimensional scaffold of fibroblastic reticular cells (FRC) but currently it is unclear how FRC influence T cell activation. Here we report that FRC lines and ex vivo FRC inhibit T cell proliferation but not differentiation. FRC share this feature with fibroblasts from non-lymphoid tissues as well as mesenchymal stromal cells. We identified FRC as strong source of nitric oxide (NO) thereby directly dampening T cell expansion as well as reducing the T cell priming capacity of DC. The expression of inducible nitric oxide synthase (iNOS) was up-regulated in a subset of FRC by both DC-signals as well as interferon-γ produced by primed CD8+ T cells. Importantly, iNOS expression was induced during viral infection in vivo in both LN FRC and DC. As a consequence, the primary T cell response was found to be exaggerated in Inos(-/-) mice. Our findings highlight that in addition to their established positive roles in T cell responses FRC and DC cooperate in a negative feedback loop to attenuate T cell expansion during acute inflammation.
Pubmed
Web of science
Open Access
Yes
Create date
05/01/2012 16:39
Last modification date
20/08/2019 16:07
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