Erythropoietin (EPO) immunoaffinity columns--a powerful tool for purifying EPO and its recombinant analogues.

Details

Serval ID
serval:BIB_E28A82A93CB0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Erythropoietin (EPO) immunoaffinity columns--a powerful tool for purifying EPO and its recombinant analogues.
Journal
Journal of Pharmaceutical and Biomedical Analysis
Author(s)
Dehnes Yvette, Lamon Séverine, Lönnberg Maria
ISSN
1873-264X[electronic], 0731-7085[linking]
Publication state
Published
Issued date
2010
Volume
53
Number
4
Pages
1028-1032
Language
english
Abstract
The sample preparation method preceding the urinary erythropoietin (EPO) doping test is based on several concentration and ultrafiltration steps. In order to improve the quality of isoelectric focusing (IEF) gel results and therefore, the sensitivity of the EPO test, new sample preparation methods relying on affinity purification were recently proposed. This article focuses on the evaluation and validation of disposable immunoaffinity columns targeting both endogenous and recombinant EPO molecules in two World Anti-Doping Agency (WADA) accredited anti-doping laboratories. The use of the columns improved the resolution of the IEF profiles considerably when compared with the classical ultrafiltration method, and the columns' ability to ensure the isoform integrity of the endogenous and exogenous EPO molecules was confirmed. Immunoaffinity columns constitute therefore a potent and reliable tool for the preparation of urine samples and their use will significantly improve the sensitivity and specificity of the actual urinary EPO test.
Keywords
Erythropoietin, Affinity purification, Isoelectric focusing, Validation, Doping control, isoelectric profiles, doping control, urine, binding, serum
Pubmed
Web of science
Create date
22/09/2010 15:05
Last modification date
20/08/2019 16:06
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