Maintenance of primary murine hepatocyte functions in multicomponent polymer capsules-in vitro cryopreservation studies.

Details

Serval ID
serval:BIB_E1DDA266CE8D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Maintenance of primary murine hepatocyte functions in multicomponent polymer capsules-in vitro cryopreservation studies.
Journal
Journal of Hepatology
Author(s)
Canaple L., Nurdin N., Angelova N., Saugy D., Hunkeler D., Desvergne B.
ISSN
0168-8278[print], 0168-8278[linking]
Publication state
Published
Issued date
2001
Volume
34
Number
1
Pages
11-18
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
BACKGROUND/AIMS: The potential of a new encapsulation system has been evaluated as an artificial housing for liver cells. METHODS: Murine hepatocytes were encapsulated in specially designed multicomponent capsules formed by polyelectrolyte complexation of sodium alginate, cellulose sulphate and poly(methylene-co-guanidine) hydrochloride, the permeability of which has previously been characterised. RESULTS: We demonstrate here the absence of cytotoxicity and the excellent biocompatibility of these capsules towards primary culture of murine hepatocytes. Experimental results demonstrated that the encapsulated hepatocytes retained their specific functions--transaminase activity, urea synthesis and protein secretion--over the first 4 days of culture in minimum medium. The cryopreservation of encapsulated hepatocytes, for periods of up to 4 months, did not alter their functional capacities, as no major differences were observed between unfrozen and frozen encapsulated cells for the functions tested. CONCLUSIONS: Because of the absence of cytotoxicity, and the ease of handling and cryopreservation, while maintaining liver specific functions, the described system appears to be valuable for murine liver cell encapsulation. It is also a promising tool for fundamental research into drug metabolism, intercellular regulation, metabolic pathways, and the establishment of banks for the supply and storage of murine hepatocytes.
Keywords
Animals, Aspartate Aminotransferases/metabolism, Cell Survival, Cells, Cultured, Cryopreservation, Hepatocytes/physiology, Liver, Artificial, Male, Mice, Polymers, Urea/metabolism
Pubmed
Web of science
Create date
24/01/2008 16:26
Last modification date
20/08/2019 17:05
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