Promoter methylation and downregulated expression of the TBX15 gene in ovarian carcinoma.

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Version: Final published version
Serval ID
serval:BIB_E144F5B34483
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Promoter methylation and downregulated expression of the TBX15 gene in ovarian carcinoma.
Journal
Oncology Letters
Author(s)
Gozzi G., Chelbi S.T., Manni P., Alberti L., Fonda S., Saponaro S., Fabbiani L., Rivasi F., Benhattar J., Losi L.
ISSN
1792-1074 (Print)
ISSN-L
1792-1074
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
12
Number
4
Pages
2811-2819
Language
english
Abstract
TBX15 is a gene involved in the development of mesodermal derivatives. As the ovaries and the female reproductive system are of mesodermal origin, the aim of the present study was to determine the methylation status of the TBX15 gene promoter and the expression levels of TBX15 in ovarian carcinoma, which is the most lethal and aggressive type of gynecological tumor, in order to determine the role of TBX15 in the pathogenesis of ovarian carcinoma. This alteration could be used to predict tumor development, progression, recurrence and therapeutic effects. The study was conducted on 80 epithelial ovarian carcinoma and 17 control cases (normal ovarian and tubal tissues). TBX15 promoter methylation was first determined by pyrosequencing following bisulfite modification, then by cloning and sequencing, in order to obtain information about the epigenetic haplotype. Immunohistochemical analysis was performed to evaluate the correlation between the methylation and protein expression levels. Data revealed a statistically significant increase of the TBX15 promoter region methylation in 82% of the tumor samples and in various histological subtypes. Immunohistochemistry showed an inverse correlation between methylation levels and the expression of the TBX15 protein. Furthermore, numerous tumor samples displayed varying degrees of intratumor heterogeneity. Thus, the present study determined that ovarian carcinoma typically expresses low levels of TBX15 protein, predominantly due to an epigenetic mechanism. This may have a role in the pathogenesis of ovarian carcinoma independent of the histological subtype.
Pubmed
Web of science
Open Access
Yes
Create date
11/10/2016 16:30
Last modification date
20/08/2019 16:05
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