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Combined use of fluorescent reporters and flow cytometry for simultaneous monitoring of bacterial growth and gene expression on plant roots
Title of the book
Handbook of hydrocarbon and lipid microbiology
Address of publication
Timmis K. N.
A protocol of a recently developed assay is presented in which FACS-based flow cytometry is used in combination with autofluorescent reporter constructs to simultaneously monitor and quantify bacterial growth (root colonization levels) and single cell activity (expression of a plant-beneficial trait) on roots of hydroponically grown plants. The experimental approach is illustrated with an example of a reporter of antifungal gene expression in a plant-beneficial strain of Pseudomonas fluorescens. It can be adapted to the study of other bacteria-root interactions.
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