Effects of apical membrane Cl(-)-formate exchange on cell volume in rabbit proximal tubule

Détails

ID Serval
serval:BIB_E0D1E9C77872
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Effects of apical membrane Cl(-)-formate exchange on cell volume in rabbit proximal tubule
Périodique
American Journal of Physiology
Auteur(s)
Schild  L., Aronson  P. S., Giebisch  G.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
03/1990
Volume
258
Numéro
3 Pt 2
Pages
F530-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
We used real-time recordings of cell volume changes to test for the role of the Cl(-)-formate exchanger in mediating NaCl entry across the apical membrane of rabbit proximal tubule cells. In the absence of extracellular Cl-, 0.5 and 5 mM formate in the tubule lumen induced an increase in cell volume of 1 and 9%, respectively. Formate-induced cell swelling was reduced by alkalinizing the tubule lumen or by addition of luminal amiloride (2 mM), indicating that the increase in cell volume results from the intracellular accumulation of Na-formate via nonionic diffusion of formic acid in parallel with Na(+)-H+ exchange. The cell volume increase induced by 0.5 mM formate was potentiated (from 1 to 4%) by Cl-, as expected for a formate-mediated stimulation of NaCl uptake via parallel Cl(-)-formate exchange and Na(+)-H+ exchange across the apical membrane. By contrast, the cell volume increase induced by 5 mM formate was attenuated (from 9 to 4%) by Cl-. The attenuating effect of Cl- on formate-induced cell swelling required the operation of the apical membrane Cl(-)-formate exchanger. The effect of 1:1 Cl(-)-formate exchange to attenuate formate-induced cell swelling can be explained if the cell possesses a volume-activated anion exit pathway, most likely at the basolateral cell membrane, that is capable of mediating the efflux of Cl- but not formate from the cell.
Mots-clé
Animals Cell Membrane/metabolism Chlorides/*metabolism/pharmacology Drug Synergism Formic Acids/*metabolism/pharmacology Hydrogen-Ion Concentration Ion Exchange Kidney Tubules, Proximal/cytology/*metabolism Osmolar Concentration Rabbits Sodium Chloride/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 13:55
Dernière modification de la notice
20/08/2019 17:05
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