Changes in nuclear 3,5,3'-triiodothyronine receptor expression in rat dorsal root ganglia and sciatic nerve during development: comparison with regeneration.

Details

Serval ID
serval:BIB_E094CA21DF04
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Changes in nuclear 3,5,3'-triiodothyronine receptor expression in rat dorsal root ganglia and sciatic nerve during development: comparison with regeneration.
Journal
European Journal of Neuroscience
Author(s)
Barakat-Walter I., Duc C., Puymirat J.
ISSN
0953-816X
Publication state
Published
Issued date
1993
Peer-reviewed
Oui
Volume
5
Number
4
Pages
319-326
Language
english
Abstract
The action of the thyroid hormones on responsive cells in the peripheral nervous system requires the presence of nuclear triiodothyronine receptors (NT3R). These nuclear receptors, including both the alpha and beta subtypes of NT3R, were visualized by immunocytochemistry with the specific 2B3 monoclonal antibody. In the dorsal root ganglia (DRG) of rat embryos, NT3R immunoreactivity was first discretely revealed in a few neurons at embryonic day 14 (E14), then strongly expressed by all neurons at E17 and during the first postnatal week; all DRG neurons continued to possess clear NT3R immunostaining, which faded slightly with age. The peripheral glial cells in the DRG displayed a short-lived NT3R immunoreaction, starting at E17 and disappearing from the satellite and Schwann cells by postnatal days 3 and 7 respectively. In the developing sciatic nerve, Schwann cells also exhibited transient NT3R immunoreactivity restricted to a short period ranging from E17 to postnatal day 10; the NT3R immunostaining of the Schwann cells vanished proximodistally along the sciatic nerve, so that the Schwann cells rapidly became free of detectable NT3R immunostaining. However, after the transection or crushing of an adult sciatic nerve, the NT3R immunoreactivity reappeared in the Schwann cells adjacent to the lesion by 2 days, then along the distal segment in which the axons were degenerating, and finally disappeared by 45 days, when the regenerating axons were allowed to re-occupy the distal segment.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Animals, Animals, Newborn, Cell Nucleus, Female, Ganglia, Spinal, Immunohistochemistry, Male, Nerve Regeneration, Rats, Rats, Wistar, Receptors, Thyroid Hormone, Sciatic Nerve
Pubmed
Web of science
Create date
30/03/2009 8:28
Last modification date
20/08/2019 16:04
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