Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA.

Détails

ID Serval
serval:BIB_E09120ADE622
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA.
Périodique
Virus Research
Auteur(s)
Plattet P., Zweifel C., Wiederkehr C., Belloy L., Cherpillod P., Zurbriggen A., Wittek R.
ISSN
0168-1702[print], 0168-1702[linking]
Statut éditorial
Publié
Date de publication
05/2004
Volume
101
Numéro
2
Pages
147-153
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Wild-type A75/17-Canine distemper virus (CDV) is a highly virulent strain, which induces a persistent infection in the central nervous system (CNS) with demyelinating disease. Wild-type A75/17-CDV, which is unable to replicate in cell lines to detectable levels, was adapted to grow in Vero cells and was designated A75/17-V. Sequence comparison between the two genomes revealed seven nucleotide differences located in the phosphoprotein (P), the matrix (M) and the large (L) genes. The P gene is polycistronic and encodes two auxiliary proteins, V and C, besides the P protein. The mutations resulted in amino acid changes in the P and V, but not in the C protein, as well as in the M and L proteins. Here, a rescue system was developed for the A75/17-V strain, which was shown to be attenuated in vivo, but retains a persistent infection phenotype in Vero cells. In order to track the recombinant virus, an additional transcription unit coding for the enhanced green fluorescent protein (eGFP) was inserted at the 3' proximal position in the A75/17-V cDNA clone. Reverse genetics technology will allow us to characterize the genetic determinants of A75/17-V CDV persistent infection in cell culture.
Mots-clé
Adaptation, Physiological, Amino Acid Substitution, Animals, Cell Line, Cercopithecus aethiops, Cloning, Molecular, Distemper Virus, Canine/genetics, Distemper Virus, Canine/growth &amp, development, Dogs, Gene Order, Genes, Reporter, Genes, Viral, Genome, Viral, Genomics, Green Fluorescent Proteins, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Mutation, Missense, Recombination, Genetic, Vero Cells, Viral Proteins/chemistry, Viral Proteins/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 10:43
Dernière modification de la notice
20/08/2019 16:04
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