BDNF stimulates expression, activity and release of tissue-type plasminogen activator in mouse cortical neurons
Details
Serval ID
serval:BIB_DFF7025BD46C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
BDNF stimulates expression, activity and release of tissue-type plasminogen activator in mouse cortical neurons
Journal
European Journal of Neuroscience
ISSN
0953-816X
Publication state
Published
Issued date
05/1999
Peer-reviewed
Oui
Volume
11
Number
5
Pages
1639-46
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor involved in neuronal development and synaptic plasticity. Although the physiological effects of BDNF have been examined in detail, target proteins which mediate its actions remain largely unknown. Here, we report that BDNF stimulates the expression of tissue-type plasminogen activator (tPA) in primary cultures of cortical neurons in a time- and concentration-dependent manner. Among the other members of the neurotrophin family, neurotrophin-4 (NT-4) and to a lesser extent neurotrophin-3 (NT-3) also increased tPA mRNA expression, while nerve growth factor (NGF) was devoid of any effect. Induction of tPA expression by BDNF is accompanied by an increase in the proteolytic activity of tPA associated with cortical neurons and a release of tPA into the extracellular space. Release of tPA induced by BDNF depends on extracellular Ca2+ since it is markedly reduced in the presence of ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA). Up-regulation of tPA expression by BDNF is followed by the induction of plasminogen activator inhibitor 2 (PAI-2), an inhibitor of tPA. Together these results suggest that activation of tPA by BDNF may contribute to structural changes associated with neuronal development or synaptic plasticity.
Keywords
Animals
Blotting, Northern
Brain-Derived Neurotrophic Factor/*pharmacology
Cells, Cultured
Cerebral Cortex/chemistry/cytology/physiology
Chelating Agents/pharmacology
Cycloheximide/pharmacology
DNA Primers
Dactinomycin/pharmacology
Egtazic Acid/pharmacology
Gene Expression/physiology
Mice
Nerve Growth Factors/pharmacology
Neuronal Plasticity/*physiology
Neurons/chemistry/drug effects/*physiology
Neuropeptides/genetics
Neurotrophin 3
Plasminogen Activator Inhibitor 1/*genetics
Plasminogen Activator Inhibitor 2/*genetics
Potassium/pharmacology
Protein Synthesis Inhibitors/pharmacology
RNA, Messenger/analysis
Serine Proteinase Inhibitors/genetics
Serpins/genetics
Tetraethylammonium/pharmacology
Pubmed
Web of science
Create date
20/01/2008 19:22
Last modification date
20/08/2019 17:04