Cytostatic activity of alveolar macrophages from smokers and nonsmokers: role of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha
Details
Serval ID
serval:BIB_DFE28D8833A8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytostatic activity of alveolar macrophages from smokers and nonsmokers: role of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha
Journal
American Journal of Respiratory Cell and Molecular Biology
ISSN
1044-1549 (Print)
Publication state
Published
Issued date
11/1994
Volume
11
Number
5
Pages
631-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Several functions of alveolar macrophages (AM) are modified by cigarette smoking. AM are the first line of defense in bronchoalveolar spaces and could be depressed in their cytotoxicity to tumor cells in smokers. An assay using A549 cells (human lung adenocarcinoma) as target cells was performed to assess cytostasis mediated by AM and their supernatants (SN) from healthy smokers (n = 8) and nonsmokers (n = 6). Contact-mediated cytostasis was decreased in AM of smokers (n = 8) relative to nonsmokers (n = 6) (22.9 +/- 5.7% versus 42.7 +/- 6.0% [+/- SEM], P < 0.04) and increased after lipopolysaccharide (LPS) stimulation in both groups (34.5 +/- 5.3% versus 46.8 +/- 5.2%, NS). Cytostasis induced by SN from nonstimulated AM was low in both groups and was still lower in smokers after LPS exposure (19.3 +/- 4.5% versus 34.5 +/- 4.8%, P < 0.04). Among cytotoxic factors produced by macrophages, interleukin (IL)-1 beta, IL-6, and tumor necrosis factor alpha (TNF alpha) may play an important role in cytostasis. Recombinant human (rH) IL-1 beta and rHTNF alpha had a moderate cytostatic activity, which was additive, whereas rHIL-6 had no significant activity on A549 cells. Bioactive IL-1 beta, IL-6, and TNF alpha were therefore measured in macrophage SN. Their levels tended to be lower in smokers than in nonsmokers and were much increased after LPS stimulation. Levels of the three cytokines were also found to correlate with each other; furthermore, a good correlation between cytokine levels in SN and cytostasis was observed.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Adenocarcinoma/immunology
Adult
Cells, Cultured
*Cytotoxicity, Immunologic
Humans
Interleukin-1/pharmacology/*physiology
Interleukin-6/pharmacology/*physiology
Lung/cytology/immunology
Lung Neoplasms/immunology
Macrophage Activation
Macrophages, Alveolar/*immunology
Smoking/*immunology
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha/pharmacology/*physiology
Pubmed
Web of science
Create date
25/01/2008 10:52
Last modification date
20/08/2019 17:04